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Title: Tailoring three-dimensional architectures by rolled-up nanotechnology for mimicking microvasculatures
Authors: Arayanarakool, RerngchaiMeyer, Anne K.Helbig, LindaSanchez, SamuelSchmidt, Oliver G.
Publishers Version: https://doi.org/10.1039/C5LC00024F
Issue Date: 2015
Published in: Lab on a Chip, Volume 15, Issue 14, Page 2981-2989
Publisher: Cambridge : Royal Society of Chemistry
Abstract: Artificial microvasculature, particularly as part of the blood–brain barrier, has a high benefit for pharmacological drug discovery and uptake regulation. We demonstrate the fabrication of tubular structures with patterns of holes, which are capable of mimicking microvasculatures. By using photolithography, the dimensions of the cylindrical scaffolds can be precisely tuned as well as the alignment and size of holes. Overlapping holes can be tailored to create diverse three-dimensional configurations, for example, periodic nanoscaled apertures. The porous tubes, which can be made from diverse materials for differential functionalization, are biocompatible and can be modified to be biodegradable in the culture medium. As a proof of concept, endothelial cells (ECs) as well as astrocytes were cultured on these scaffolds. They form monolayers along the scaffolds, are guided by the array of holes and express tight junctions. Nanoscaled filaments of cells on these scaffolds were visualized by scanning electron microscopy (SEM). This work provides the basic concept mainly for an in vitro model of microvasculature which could also be possibly implanted in vivo due to its biodegradability.
Keywords: astrocyte; biodegradable implant; cell culture; chemistry; cytology; devices; endothelium cell; human; microvasculature; nanotechnology; particle size; porosity; scanning electron microscopy; surface property; tissue scaffold
DDC: 620
License: CC BY-NC 3.0 Unported
Link to License: https://creativecommons.org/licenses/by-nc/3.0/
Appears in Collections:Ingenieurwissenschaften



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