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Title: Symmetric exchange of multi-protein building blocks between stationary focal adhesions and the cytosol
Authors: Hoffmann, Jan-ErikFermin, YessicaStricker, Ruth LOIckstadt, KatjaZamir, Eli
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Issue Date: 2014
Published in: eLife, Volume 3
Publisher: Cambridge : eLife Sciences Publications
Abstract: How can the integrin adhesome get self-assembled locally, rapidly, and correctly as diverse cell-matrix adhesion sites? Here, we investigate this question by exploring the cytosolic state of integrin-adhesome components and their dynamic exchange between adhesion sites and cytosol. Using fluorescence cross-correlation spectroscopy (FCCS) and fluorescence recovery after photobleaching (FRAP) we found that the integrin adhesome is extensively pre-assembled already in the cytosol as multi-protein building blocks for adhesion sites. Stationary focal adhesions release symmetrically the same types of protein complexes that they recruit, thereby keeping the cytosolic pool of building blocks spatiotemporally uniform. We conclude a model in which multi-protein building blocks enable rapid and modular self-assembly of adhesion sites and symmetric exchange of these building blocks preserves their specifications and thus the assembly logic of the system.
Keywords: alpha actinin; alpha parvin; focal adhesion kinase; glycoprotein; integrin adhesome; integrin linked kinase; paxillin; peptides and proteins; protein SH2; talin; tensin; unclassified drug; vasodilator stimulated phosphoprotein; vinculin; zyxin
DDC: 570
License: CC BY 3.0 Unported
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