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Title: Bifunctional hydrogels containing the laminin motif IKVAV promote neurogenesis
Authors: Farrukh, AleezaOrtega, FelipeFan, WenqiangMarichal, NicolásPaez, Julieta I.Berninger, Benediktdel Campo, AránzazuSalierno, Marcelo J.
Publishers Version: https://doi.org/10.1016/j.stemcr.2017.09.002
Issue Date: 2017
Published in: Stem Cell Reports , Volume 9, Page 1432-1440
Publisher: Amsterdam : Elsevier
Abstract: Engineering of biomaterials with specific biological properties has gained momentum as a means to control stem cell behavior. Here, we address the effect of bifunctionalized hydrogels comprising polylysine (PL) and a 19-mer peptide containing the laminin motif IKVAV (IKVAV) on embryonic and adult neuronal progenitor cells under different stiffness regimes. Neuronal differentiation of embryonic and adult neural progenitors was accelerated by adjusting the gel stiffness to 2 kPa and 20 kPa, respectively.While gels containing IKVAV or PL alone failed to support long-term cell adhesion, in bifunctional gels, IKVAV synergized with PL to promote differentiation and formation of focal adhesions containing b1-integrin in embryonic cortical neurons. Furthermore, in adult neural stem cell culture, bifunctionalized gels promoted neurogenesis via the expansion of neurogenic clones. These data highlight the potential of synthetic matrices to steer stem and progenitor cell behavior via defined mechano-adhesive properties.
DDC: 570
License: This document may be downloaded, read, stored and printed for your own use within the limits of § 53 UrhG but it may not be distributed via the internet or passed on to external parties.
Dieses Dokument darf im Rahmen von § 53 UrhG zum eigenen Gebrauch kostenfrei heruntergeladen, gelesen, gespeichert und ausgedruckt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden.
Appears in Collections:Biowissenschaften



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