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Title: Red blood cell passage of small capillaries is associated with transient Ca2+-mediated adaptations
Authors: Danielczok, Jens G.Terriac, EmmanuelHertz, LauraPetkova-Kirova, PolinaLautenschläger, FranziskaLaschke, Matthias W.Kaestner, Lars
Publishers Version: https://doi.org/10.3389/fphys.2017.00979
Issue Date: 2017
Published in: Frontiers in Physiology , Volume 8
Publisher: Lausanne : Frontiers Media
Abstract: When red blood cells (RBCs) pass constrictions or small capillaries they need to pass apertures falling well below their own cross section size. We used different means of mechanical stimulations (hypoosmotic swelling, local mechanical stimulation, passing through microfluidic constrictions) to observe cellular responses of human RBCs in terms of intracellular Ca2+-signaling by confocal microscopy of Fluo-4 loaded RBCs.We were able to confirm ourin vitro results in a mouse dorsal skinfold chamber model showing a transiently increased intracellular Ca2+ when RBCs were passing through small capillaries in vivo. Furthermore, we performed the above-mentioned in vitro experiments as well as measurements of RBCs filterability under various pharmacological manipulations (GsMTx-4, TRAM-34) to explore the molecular mechanism of the Ca2+-signaling. Based on these experiments we conclude that mechanical stimulation of RBCs activates mechano-sensitive channels most likely Piezo1. This channel activity allows Ca2+ to enter the cell, leading to a transient activation of the Gardos-channel associated with K+, Cl−, and water loss, i.e., with a transient volume adaptation facilitating the passage of the RBCs through the constricti on.
Keywords: RBC deformation; Piezo1; hSK4 (KCNN4); Ca2+ imaging; microfluidics; dorsal skinfold chamber
DDC: 570
License: This document may be downloaded, read, stored and printed for your own use within the limits of § 53 UrhG but it may not be distributed via the internet or passed on to external parties.
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Appears in Collections:Biowissenschaften



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