Please use this identifier to cite or link to this item: https://oar.tib.eu/jspui/handle/123456789/4784
Files in This Item:
File SizeFormat 
Reconstitution of immune cell interactions in free-standing membranes.pdf2,85 MBAdobe PDFView/Open
Title: Reconstitution of immune cell interactions in free-standing membranes
Authors: Jenkins, EdwardSantos, Ana M.Felce, James HHatherley, DeborahDustin, Michael L.Davis, Simon J.Eggeling, ChristianSezgin, Erdinc
Publishers Version: https://doi.org/10.1242/jcs.219709
Issue Date: 2019
Published in: Journal of Cell Science 132 (2019)
Publisher: Cambridge : Company of Biologists
Abstract: The spatiotemporal regulation of signalling proteins at the contacts formed between immune cells and their targets determines how and when immune responses begin and end. Therapeutic control of immune responses therefore relies on thorough elucidation of the molecular processes occurring at these interfaces. However, the detailed investigation of each component's contribution to the formation and regulation of the contact is hampered by the complexities of cell composition and architecture. Moreover, the transient nature of these interactions creates additional challenges, especially in the use of advanced imaging technology. One approach that circumvents these problems is to establish in vitro systems that faithfully mimic immune cell interactions, but allow complexity to be ‘dialled-in’ as needed. Here, we present an in vitro system that makes use of synthetic vesicles that mimic important aspects of immune cell surfaces. Using this system, we began to explore the spatial distribution of signalling molecules (receptors, kinases and phosphatases) and how this changes during the initiation of signalling. The GUV/cell system presented here is expected to be widely applicable.The spatiotemporal regulation of signalling proteins at the contacts formed between immune cells and their targets determines how and when immune responses begin and end. Therapeutic control of immune responses therefore relies on thorough elucidation of the molecular processes occurring at these interfaces. However, the detailed investigation of each component's contribution to the formation and regulation of the contact is hampered by the complexities of cell composition and architecture. Moreover, the transient nature of these interactions creates additional challenges, especially in the use of advanced imaging technology. One approach that circumvents these problems is to establish in vitro systems that faithfully mimic immune cell interactions, but allow complexity to be ‘dialled-in’ as needed. Here, we present an in vitro system that makes use of synthetic vesicles that mimic important aspects of immune cell surfaces. Using this system, we began to explore the spatial distribution of signalling molecules (receptors, kinases and phosphatases) and how this changes during the initiation of signalling. The GUV/cell system presented here is expected to be widely applicable.
Keywords: Immune signalling; Immune synapse; In vitro reconstitution; Model membranes; Giant unilamellar vesicles
DDC: 570
License: CC BY 3.0 Unported
Link to License: https://creativecommons.org/licenses/by/3.0/
Appears in Collections:Ingenieurwissenschaften



This item is licensed under a Creative Commons License Creative Commons