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Title: Effects of new beta-type Ti-40Nb implant materials, brain-derived neurotrophic factor, acetylcholine and nicotine on human mesenchymal stem cells of osteoporotic and non osteoporotic donors
Authors: Kauschke, V.Gebert, A.Calin, M.Eckert, J.Scheich, S.Heiss, C.Lips, K.S.
Publishers Version: https://doi.org/10.1371/journal.pone.0193468
Issue Date: 2018
Published in: PLoS ONE Vol. 13 (2018), No. 2
Publisher: San Francisco, CA : Public Library of Science (PLoS)
Abstract: Introduction Treatment of osteoporotic fractures is still challenging and an urgent need exists for new materials, better adapted to osteoporotic bone by adjusted Young’s modulus, appropriate surface modification and pharmaceuticals. Materials and methods Titanium-40-niobium alloys, mechanically ground or additionally etched and titanium-6-alu-minium-4-vanadium were analyzed in combination with brain-derived neurotrophic factor, acetylcholine and nicotine to determine their effects on human mesenchymal stem cells in vitro over 21 days using lactate dehydrogenase and alkaline phosphatase assays, live cell imaging and immunofluorescence microscopy. Results Cell number of human mesenchymal stem cells of osteoporotic donors was increased after 14 d in presence of ground titanium-40-niobium or titanium-6-aluminium-4-vanadium, together with brain-derived neurotrophic factor. Cell number of human mesenchymal stem cells of non osteoporotic donors increased after 21 d in presence of titanium-6-aluminium-4-vanadium without pharmaceuticals. No significant increase was measured for ground or etched titanium-40-niobium after 21 d. Osteoblast differentiation of osteoporotic donors was significantly higher than in non osteoporotic donors after 21 d in presence of etched, ground titanium-40-niobium or titanium-6-aluminium-4-vanadium accompanied by all pharmaceuticals tested. In presence of all alloys tested brain-derived neurotrophic factor, acetylcholine and nicotine increased differentiation of cells of osteoporotic donors and accelerated it in non osteoporotic donors. Conclusion We conclude that ground titanium-40-niobium and brain-derived neurotrophic factor might be most suitable for subsequent in vivo testing.
Keywords: acetylcholine; alkaline phosphatase; brain derived neurotrophic factor; lactate dehydrogenase; nicotine; titanium; titanium 6 aluminum 4 vanadium; titanium niobium alloy 40; unclassified drug; acetylcholine; alkaline phosphatase; alloy; brain derived neurotrophic factor; nicotine; titanium-niobium alloy; adult; aged; Article; cell count; cell differentiation; controlled study; donor; drug activity; enzyme assay; female; human; human cell; human tissue; immunofluorescence microscopy; implantation; in vitro study; live cell imaging; major clinical study; male; mesenchymal stem cell; non osteoporosis; osteoblast; osteoporosis; cell count; cell differentiation; cytology; drug effect; drug interaction; mesenchymal stroma cell; metabolism; middle aged; molecular imaging; osteoporosis; pathology; very elderly; Acetylcholine; Adult; Aged; Aged, 80 and over; Alkaline Phosphatase; Alloys; Brain-Derived Neurotrophic Factor; Cell Count; Cell Differentiation; Drug Interactions; Female; Humans; Male; Mesenchymal Stromal Cells; Middle Aged; Molecular Imaging; Nicotine; Osteoporosis
DDC: 610
License: CC BY 4.0 Unported
Link to License: https://creativecommons.org/licenses/by/4.0/
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