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Title: Targeted delivery of functionalized PLGA nanoparticles to macrophages by complexation with the yeast Saccharomyces cerevisiae
Authors: Kiefer, R.Jurisic, M.Dahlem, C.Koch, M.Schmitt, M.J.Kiemer, A.K.Schneider, M.Breinig, F.
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Issue Date: 2020
Published in: Biotechnology and Bioengineering Vol. 117 (2020), No. 3
Publisher: Chichester : John Wiley and Sons Ltd
Abstract: Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering the body. Therefore, we investigated the interaction of biodegradable surface-modified poly(lactic-co-glycolic acid) (PLGA) particles with yeast cells to overcome the unspecificity of the particulate carriers. Cells of different Saccharomyces cerevisiae strains were characterized regarding their interaction with PLGA-NPs under isotonic and hypotonic conditions. The particles were shown to efficiently interact with yeast cells leading to stable NP/yeast-complexes allowing to associate or even internalize compounds. Notably, applying those complexes to a coculture model of HeLa cells and macrophages, the macrophages were specifically targeted. This novel nano-in-micro carrier system suggests itself as a promising tool for the delivery of biologically active agents into phagocytic cells combining specificity and efficiency.
Keywords: drug delivery; immunotherapy; nanomedicine; phagocytosis; yeast; Antigen-antibody reactions; Controlled drug delivery; Cytology; Drug delivery; Lanthanum compounds; Macrophages; Medical nanotechnology; Nanoparticles; Yeast; immunotherapy; Nanoparticle (NPs); phagocytosis; PLGA nanoparticles; Poly(lactic-co-glycolic acid); Saccharomyces cerevisiae strains; Targeted delivery; Yeast Saccharomyces cerevisiae; Targeted drug delivery; carrier protein; chitosan; drug carrier; hypotonic solution; isotonic solution; nanoparticle; nucleic acid; polyglactin; Article; cell culture; cell viability; chemical interaction; clinical assessment; coculture; complex formation; controlled study; drug delivery system; female; HeLa cell line; human; human cell; macrophage; outcome assessment; particle size; Saccharomyces cerevisiae; surface property; yeast cell
DDC: 570
License: CC BY 4.0 Unported
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Appears in Collections:Biowissenschaften

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