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Title: A novel, low-volume method for organ culture of embryonic kidneys that allows development of cortico-medullary anatomical organization
Authors: Sebinger, D.D.R.Unbekandt, M.Ganeva, V.V.Ofenbauer, A.Werner, C.Davies, J.A.
Publishers Version: https://doi.org/10.1371/journal.pone.0010550
Issue Date: 2010
Published in: PLoS ONE Vol. 5 (2010), No. 5
Publisher: San Francisco, Calif. : Public Library of Science
Abstract: Here, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods. Organ culture is a powerful technique for studying renal development. It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1-3 mls) of culture medium, which can make high-throughput screens expensive, they require porous (filter) substrates which are difficult to modify chemically, and the organs produced do not achieve good cortico-medullary zonation. Here, we present a technique of growing kidney rudiments in very low volumes of medium-around 85 microliters-using silicone chambers. In this system, kidneys grow directly on glass, grow larger than in conventional culture and develop a clear anatomical cortico-medullary zonation with extended loops of Henle. © 2010 Sebinger et al.
Keywords: animal tissue; article; branching morphogenesis; cell count; cell size; concentration process; controlled study; culture medium; embryo; embryo culture; embryo development; Henle loop; intermethod comparison; kidney cell culture; kidney cortex; kidney development; kidney medulla; mouse; nephron; nonhuman; organ culture technique; primordium; ureter; animal; animal embryo; cell death; cell proliferation; culture medium; cytology; drug effect; growth, development and aging; histology; kidney cortex; kidney medulla; methodology; morphogenesis; organ culture technique; physiological stress; prenatal development; surface tension; silicone derivative; Animals; Cell Death; Cell Proliferation; Culture Media; Embryo, Mammalian; Kidney Cortex; Kidney Medulla; Mice; Morphogenesis; Nephrons; Organ Culture Techniques; Silicones; Stress, Physiological; Surface Tension
DDC: 570
License: CC BY 3.0 Unported
Link to License: https://creativecommons.org/licenses/by/3.0/
Appears in Collections:Biowissenschaften



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