Please use this identifier to cite or link to this item: https://oar.tib.eu/jspui/handle/123456789/5514
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dc.rights.licenseCC BY 3.0 Unportedger
dc.contributor.authorSebinger, D.D.R.-
dc.contributor.authorUnbekandt, M.-
dc.contributor.authorGaneva, V.V.-
dc.contributor.authorOfenbauer, A.-
dc.contributor.authorWerner, C.-
dc.contributor.authorDavies, J.A.-
dc.date.accessioned2020-08-13T10:35:46Z-
dc.date.available2020-08-13T10:35:46Z-
dc.date.issued2010-
dc.identifier.urihttp://dx.doi.org/10.34657/4143-
dc.identifier.urihttps://oar.tib.eu/jspui/handle/123456789/5514
dc.description.abstractHere, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods. Organ culture is a powerful technique for studying renal development. It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1-3 mls) of culture medium, which can make high-throughput screens expensive, they require porous (filter) substrates which are difficult to modify chemically, and the organs produced do not achieve good cortico-medullary zonation. Here, we present a technique of growing kidney rudiments in very low volumes of medium-around 85 microliters-using silicone chambers. In this system, kidneys grow directly on glass, grow larger than in conventional culture and develop a clear anatomical cortico-medullary zonation with extended loops of Henle. © 2010 Sebinger et al.eng
dc.language.isoeng-
dc.publisherSan Francisco, Calif. : Public Library of Science-
dc.relation.ispartofseriesPLoS ONE Vol. 5 (2010), No. 5-
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/ger
dc.subjectanimal tissueeng
dc.subjectarticleeng
dc.subjectbranching morphogenesiseng
dc.subjectcell counteng
dc.subjectcell sizeeng
dc.subjectconcentration processeng
dc.subjectcontrolled studyeng
dc.subjectculture mediumeng
dc.subjectembryoeng
dc.subjectembryo cultureeng
dc.subjectembryo developmenteng
dc.subjectHenle loopeng
dc.subjectintermethod comparisoneng
dc.subjectkidney cell cultureeng
dc.subjectkidney cortexeng
dc.subjectkidney developmenteng
dc.subjectkidney medullaeng
dc.subjectmouseeng
dc.subjectnephroneng
dc.subjectnonhumaneng
dc.subjectorgan culture techniqueeng
dc.subjectprimordiumeng
dc.subjecturetereng
dc.subjectanimaleng
dc.subjectanimal embryoeng
dc.subjectcell deatheng
dc.subjectcell proliferationeng
dc.subjectculture mediumeng
dc.subjectcytologyeng
dc.subjectdrug effecteng
dc.subjectgrowth, development and agingeng
dc.subjecthistologyeng
dc.subjectkidney cortexeng
dc.subjectkidney medullaeng
dc.subjectmethodologyeng
dc.subjectmorphogenesiseng
dc.subjectorgan culture techniqueeng
dc.subjectphysiological stresseng
dc.subjectprenatal developmenteng
dc.subjectsurface tensioneng
dc.subjectsilicone derivativeeng
dc.subjectAnimalseng
dc.subjectCell Deatheng
dc.subjectCell Proliferationeng
dc.subjectCulture Mediaeng
dc.subjectEmbryo, Mammalianeng
dc.subjectKidney Cortexeng
dc.subjectKidney Medullaeng
dc.subjectMiceeng
dc.subjectMorphogenesiseng
dc.subjectNephronseng
dc.subjectOrgan Culture Techniqueseng
dc.subjectSiliconeseng
dc.subjectStress, Physiologicaleng
dc.subjectSurface Tensioneng
dc.subject.ddc570-
dc.titleA novel, low-volume method for organ culture of embryonic kidneys that allows development of cortico-medullary anatomical organizationeng
dc.typearticle-
dc.typeText-
dc.description.versionpublishedVersioneng
local.accessRightsopenAccess-
wgl.contributorIPFger
wgl.subjectBiowissenschaften/Biologieger
wgl.typeZeitschriftenartikelger
dc.bibliographicCitation.firstPagee10550-
dc.bibliographicCitation.volume5-
dc.bibliographicCitation.issue5-
dc.relation.doihttps://doi.org/10.1371/journal.pone.0010550-
dc.relation.issn1932-6203-
dcterms.bibliographicCitation.journalTitlePLoS ONE-
local.identifier.doihttp://dx.doi.org/10.34657/4143-
Appears in Collections:Biowissenschaften

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