Please use this identifier to cite or link to this item: https://oar.tib.eu/jspui/handle/123456789/6052
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dc.rights.licenseCC BY-NC-ND 4.0 Unporteden
dc.contributor.authorNair, Roshna V.-
dc.contributor.authorZhao, Shifang-
dc.contributor.authorTerriac, Emmanuel-
dc.contributor.authorLautenschläger, Franziska-
dc.contributor.authorHetmanski, Joseph H.R.-
dc.contributor.authorCaswell, Patrick T.-
dc.contributor.authordel Campo, Aranzazu-
dc.date.accessioned2021-01-06T11:40:32Z-
dc.date.available2021-01-06T11:40:32Z-
dc.date.issued2020-
dc.identifier.urihttp://dx.doi.org/10.34657/4681-
dc.identifier.urihttps://oar.tib.eu/jspui/handle/123456789/6052
dc.description.abstractThe study of the actin cytoskeleton and related cellular processes requires tools to specifically interfere with actin dynamics in living cell cultures, ideally with spatiotemporal control and compatible with real time imaging. A phototriggerable derivative of the actin disruptor Cytochalasin D (CytoD) is described and tested here. It includes a nitroveratryloxycarbonyl (Nvoc) photoremovable protecting group (PPG) at the hydroxyl group at C7 of CytoD. The attachment of the PPG renders Nvoc-CytoD temporarily inactive, and enables light-dosed delivery of the active drug CytoD to living cells. This article presents the full structural and physicochemical characterization, the toxicity analysis. It is complemented with biological tests to show the time scales (seconds) and spatial resolution (cellular level) achievable with a UV source in a regular microscopy setupeng
dc.language.isoengen
dc.publisherWashington, D.C. : American Chemical Societyen
dc.relation.ispartofseriesChemRxiven
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectpreprinteng
dc.subjectNvoc-CytoDeng
dc.subjectCytochalasin Deng
dc.subject.ddc540en
dc.titlePossibilities and Limitations of Photoactivatable Cytochalasin D for the Spatiotemporal Regulation of Actin Dynamicsen
dc.typearticleen
dc.typeText-
dc.description.versionsubmittedVersion-
local.accessRightsopenAccessen
wgl.contributorINMen
wgl.subjectChemieen
wgl.typeZeitschriftenartikelen
dc.relation.doihttps://doi.org/10.26434/chemrxiv.12609545.v1-
dcterms.bibliographicCitation.journalTitleChemRxiven
local.identifier.doihttp://dx.doi.org/10.34657/4681-
Appears in Collections:Chemie

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